Young forces in the Microbiological laboratory

It’s always dynamic in our labs, so this week was’t any different. Karmen Amižić and Merima Zulić, pupils of the Medical School in Split, joined our daily activities since they expressed their desire to get acquainted with the actual laboratory work that takes place at our Institute as part of the GRAPE-PATH3 project. During their stay, they learned to work with basic tools in a Microbiological laboratory. They also learned advanced spectrophotometric methods that are used to determine the concentration of photosynthetic pigments in samples of healthy and virus-infected grapevines.

Thank you Karmen and Merima for your help. We hope you had a pleasant and  educative stay with us and wish you prosperous education in the future. We have no doubt you will be an excellent students!

Disemination of GRAPE-PATH3 results on Symposium on Agriculture

We participated in the 56th Croatian and 16th International Symposium on Agriculture, which was held in Vodice from 6 to 10 September 2021. At the symposium we presented some of the results obtained on the GRAPE-PATH3 project. An oral presentation entitled “Presence of the virus in Dalmatian indigenous grapevine varieties” was held by Mate Čarija as part of the Viticulture and Enology section.

It was great to see in person and talk with the colleagues after long time!

Disemination of GRAPE-PATH3 results on World Microbe Forum

We participated to on-line organized World Microbe Forum (20-24. June) by American Society for MIcrobiology and Federation of European Microbiological Societies. In the form of iPoster entitled: GLRaV-3 Genetic Variants in Indigenous Vines of Mediterranean Croatia by Katarina Hančević, Pasquale Saldarelli, Mate Čarija, Silvija Černi , Goran Zdunić, Ana Mucalo and Tomislav Radić we presented spme of the results gained on the GRAPE-PATH3 project.

Friday Seminars at 13

Two more oral presentations of the results gained on the GRAPE-PATH3 project were presented as a part of a seminar organized by the Institute of Adriatic Crops, entitled “Friday Seminars at 13”. First lecture on Friday, 21.05. was held by PhD student Mate  Čarija, under the title: “Sanitary status of Plavac Mali and genetic variability of Grapevine leafroll-associated virus 3”, and the second entitled “GRAPE-PATH3, how far we have come” was held by Dr. Katarina Hančević on 18.06.2021. You can find more about the topics and content of the lectures at the following link: http://novosti.krs.hr/category/pu13/

Science Festival 2021

Together with colleagues, we actively participated in the Science Festival, which took place this year from 10 to 15 May. At the Institute for Adriatic Crops, we have prepared a virtual workshop through the ZOOM platform, on Friday, May 14th. Through joint activities: Mediterranean agriculture and forestry – research at the Institute for Adriatic Crops we tried to bring science closer to the children and adults by helping them understand how important is the science we conduct at the Institute in today’s society and human lives. Our team participated with the subtopic “Grapevine viruses” where Mate presented about the knowledge and importance of the viruses for the grapevine growing, and the importance of the research we perform on the project GRAPE-PATH3!

Master thesis „Influence of Grapevine leafroll-associated virus 3 on stress indicators in grapevine“ by Emanuel Gaši

On May 13, 2021, Emanuel Gaši successfully defended his Master thesis: ” Influence of Grapevine leafroll-associated virus 3 on stress indicators in grapevine ” at the Faculty of Science in Zagreb. His supervisor was Prof. Sandra Radić Brkanac, project associate, under whose mentorship, Emanuel made laboratory analyzes of grapevine samples from our experiment and prepared a thesis. The members of the reviewer commission evaluated the work with the highest grade. Congratulations to Emanuel on a great job and presentation, as well as to his mentor! This is also the first Master thesis made as part of the project, and we hope this is only the first one arose from the GRAPE-PATH3 project!

Training on Real-time PCR detection and quantification goes on

In the period from 19 to 24 April, the second part of the training of doctoral student Mate Čarija was held at the Department of Biology of the Faculty of Science in Zagreb, on the detection and quantification of various grapevine viruses by Real-time PCR. We detected viruses at the second time point in 3 species of indicator plants and  cv. Tribidrag, all of which were infected with field isolates, of previously determined viral composition. We also quantified GLRaV-3 in these infections. As during the first part of the training, the training was led by Dr. sc. Silvija Černi, collaborator on the GRAPE-PATH3 project.

Disemination of GRAPE-PATH3 results on IAPV conference

Our assistant on the GRAPE-PATH3 project, Mate Čarija participated on this year’s online conference International Advances in Plant Virology organized by Association of Applied Biologists stationed in England. Title of the poster presentation was: Screening for grapevine viruses and GLRaV-3 genetic diversity in the most appreciated Croatian red indigenous cultivar Plavac Mali. The abstract will be published in this year’s conference book. For those interested in lectures which occurred on the conference, you can find them on the following link: https://www.youtube.com/channel/UC3yiNfbNqoKPqR15zqxTwAg

First results on virus detection and quantification gained by real-time PCR method

With Mate Čarija going to training at  doc.dr. sc. Silvije Černi, project associate from the Biology Department of the Faculty of Science in Zagreb, we have started a new activity on the project. Real-time PCR is a reliable and accurate method for the determination and quantification of specific DNA molecules. In our team, we used this method for the first time to detect specific parts of viral DNA and to quantify GLRaV-3. The training was conducted on the Applied Biosystems 7300 Real-Time PCR System and lasted from 17 to 23 February. As part of the training, one set of plant samples from the experiment was performed.

Published scientific paper: “Predominance and Diversity of GLRaV-3 in Native Vines of Mediterranean Croatia” in the journal Plants

The first scientific paper within the GRAPE-PATH3 project on the topic of the prevalence and frequency of the virus in indigenous grapevine varieties and the genetic variability of the GLRaV-3 virus was published in the journal Plants (IF=3.935; Plant Sciences). The full paper is available at:

https://doi.org/10.3390/plants10010017