In the period from 19 to 24 April, the second part of the training of doctoral student Mate Čarija was held at the Department of Biology of the Faculty of Science in Zagreb, on the detection and quantification of various grapevine viruses by Real-time PCR. We detected viruses at the second time point in 3 species of indicator plants and  cv. Tribidrag, all of which were infected with field isolates, of previously determined viral composition. We also quantified GLRaV-3 in these infections. As during the first part of the training, the training was led by Dr. sc. Silvija Černi, collaborator on the GRAPE-PATH3 project.