Short training of PhD Mate Čarija

Mate Čarija was in the short scientific training from 17.06.-30.06. 2019 at the Institute for Sustainable Plant Protection in Bari, Italy. The host was prof. dr. sc. Pasquale Saldarelli, also a collaborator on the project.

While we in Croatia were celebrating two holidays during that period, Mate was in the Laboratory of prof. Saldarelli learning and adopting the Real-Time PCR method, advanced analytical detection and quantification method for grapevine viruses. In the second week of his stay in Bari, Mate was hosted at the Agritest Laboratory to perform ELISA on  the Croatian autochthonous grapevine samples.

Bravo Mate for great learning and results and we owe you two days off the work :-)!

Internal methodological workshop

We attended an internal methodological workshop at the Faculty of Science  in Zagreb. At the workshop, we were theoretically and practically introduced with the method of  SSCP (Single-Strand Conformation Polymorphism)  used to determine genetic variants present in the sample. The method was used to determine the genetic structure of GLRaV.-3 virus in grapevine plants.  The workshop has lasted for 5 days and was led by PhD Silvija Černi, associate on the project.

The program of the workshop was adapted on an everyday basis in accordance with the protocol for the implementation of the SSCP method and results obtained.

Plant production and preparation for biological indexing of GLRaV-3

Set of virus-free cuttings of indicator plants were put to rooting for the purpose of GLRaV-3 biological detection and characterization.  Indexing plants includes following grapevine cultivars: Pinot noir, Merlot and Cabernet franc. Cuttings of autochthonous cultivar Tribidrag were also put to rooting so the symptoms occurrence as well as the morphological, physiological, biochemical changes after the virus infection could  be observed and described. Special attention will be paid on the interaction between virus and host plants by exploring the dynamic of virus replication throughout the infection processes.

Some rooted seedlings were already transplanted into pots and placed in a greenhouse chamber, under the proper irrigation, nutrition and protection regime.

First results on grapevine virus detection

In the Laboratory for Molecular Characterization at the Institute for Adriatic Crops and Karst Reclamation we have  introduced a protocol for the detection of grapevine viruses  so we have successfully detected all 5 viruses included in the EU directive.  European Commission directive 2005/43/EC considers infectious degeneration [Grapevine fanleaf virus(GFLV), Arabis mosaic virus (ArMV)],  leafroll [Grapevine leafroll associated virus 1 (GLRaV-1), Grapevine leafroll associated virus 3 (GLRaV-3)], fleck [Grapevine fleck virus  (GFkV) only for rootstocks] and their causal viruses, as harmful diseases  whose  presence  must  be monitored and if found,  excluded  from  nursery stocks upon implementation of national certification schemes. Plenty of viruses in our autochtonous Croatian grapevine cultivars!

Lecture at the Science Festival

Mate Čarija, MSc in hort. held a lecture “All colors of Grapevine Viruses” at the Split Science Festival. Mate spoke in a scientifically-popular way about the consequences of different viruses on grapevine plants  and the ways of preventing the infection. The lecture was held on April 10, 2019 in the Library of the Institute for Adriatic Crops and Karst Reclamation in Split. The lecture was also the first presentation by Mate as a collaborator on the GRAPE-PATH3 project. It was a very educational, interesting and colorful lecture!

For more information, you can download the program at

A new staff member – PhD student Mate Čarija

Mate Čarija, MSc in horticulture,  has started  working  at the Institute for Adriatic Crops and Karst Reclamation at the Department of Plant Sciences within the project : “Young  researchers’  career  development  project –training  ofdoctoral students” of the Croatian Science Foundation. Graduated from the Faculty of Agriculture, University of Zagreb, in 2019, he was studiing viticulture and enology. Already during the Study, Mate worked actively in the field of plant virology, spending  a month at the Institute National De La Recherche Agronomique, Montpellier, France. His research work will be focused on  the  pathogenic potential of GLRaV – 3 virus and its interaction with plant host.

Mate, welcome!

Kick-off meeting

Kick off meeting was held on March 11 and 12, 2019 at the Institute’s lecture hall. Project objectives, research methods and expected results were presented. Research plan were discussed as well as activities per each project associate. The key stages for the successful implementation of the project were defined.


Monday (start at 9:00)

1. Project presentation

2. Associates activity review

3. Discussion

Tuesday (start at 9:00)

1.Lecture of prof. Sandre Radić Brkanac entitled “Mechanisms of Plant Response to   High Salinity”

2. Work in the working group

3. Lunch