First results on virus detection and quantification gained by real-time PCR method

With Mate Čarija going to training at  doc.dr. sc. Silvije Černi, project associate from the Biology Department of the Faculty of Science in Zagreb, we have started a new activity on the project. Real-time PCR is a reliable and accurate method for the determination and quantification of specific DNA molecules. In our team, we used this method for the first time to detect specific parts of viral DNA and to quantify GLRaV-3. The training was conducted on the Applied Biosystems 7300 Real-Time PCR System and lasted from 17 to 23 February. As part of the training, one set of plant samples from the experiment was performed.